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We used different protocols and DNA polymerases for different goals and type of PCRs. The different type of PCRs are summarized
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How to Calculate the Tm and Ta of the primers after adding attB sites? | ResearchGate
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Comparison of DNA Quantification Methods for Next Generation Sequencing | Scientific Reports
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Primer Dilution and Resuspension Calculator | IDT
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Nucleic Acid Design Applications Polymerase Chain Reaction (PCR) Calculating Melting Temperature (Tm) PCR Primers Design. - ppt download
